In vitro antifungal activity of lime peels extract (Citrus aurantifolia (Cristm.) Swingle.) against Fusarium oxysporum Schlecht. em. Snyd. & Hans.

Rizki Wuri Widaryuni, Oktira Roka Aji

Abstract


Fusarium wilt in plants is caused by  Fusarium oxysporum. Fusarium wilt disease prevention using synthetic fungicides cause various problems in the environment and human health, it is necessary to change to natural fungicides like lime peels. This study was conducted to determine the percentage of antifungal activity at the optimum concentration of ethanol extract of lime peel (Citrus aurantifolia) to inhibit the growth of Fusarium oxysporum. Lime peel extract was obtained from the maceration process using 96% ethanol solvent. Antifungal activity test uses a food poisoning method with mycelium diameter parameters that grow on the media. The concentration of ethanol extract of lime peels tested was 0%, 15%, 30%, 45%, and 60%, and the positive control used was synthetic antracol fungicide 0.3%. The results showed the optimum concentration of ethanol extract of lime peel to inhibit the growth of Fusarium oxysporum was a concentration of 15% with a percentage of antifungal activity is 56.34%. This is because the concentration is the lowest concentration that can inhibit the growth of Fusarium oxysporum with a strong level of activity.

Keywords


Citrus aurantifolia; Fusarium oxysporum; Antifungal activity

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References


Adegoke, A.A. dan Adebayo-tayo, B.C. 2009. Antibacterial Activity and Phytochemical Analysis of Leaf Extracts of Lasienthera africanum. African Journal of Biotechnology, 3(3): 156.

Aniszewki, T. 2007. Alkaloid-secrets of Life. Amsterdam : Elsevier.

Cai, R., Hu, M., Zhang, Y., Niu, C., Yue, T., Yuan, Y., and Wang, Z. 2019. Antifungal activity and mechanism of citral, limonene, and eugenol against Zygosaccharomyces rouxii. LWT Food Science and Technology, 109: 50-56.

Fouillaud, M., Venkatachalam, M., Valenciennes, E., Caro, Y., dan Dufosse, L. 2016. Anthraquinones and Derivatives from Marine-Derived Fungi: Structural Diversity and Selected Biological Activities. Marine Drugs, 14(4): 64.

Kadja, D.H. 2013. ‘Biological Control of Fusarium sp. using Rhizobacteria’. Tesis. Faculty of Agriculture, Nusa Cendana University, Kupang.

Khotimah, K., Rahmawati, and Mukarlina. 2017. Antifungal activity of ethanolic extract of Citrus nobilis var. microcarpa peels from its stem base, against Phytophthora sp. Im5. Protobiont ,6 (3) : 188 – 193.

Kidd, S., Halliday, C., Alexiou, H., dan Ellis, D. 2016. Description of Medical Fungi. Australia: National Mycology Reference Centre Microbiology & Infectious Diseases.

Novriyanti, E, Santosa, E, Syafii, W, Turjaman, M, & Sitepu, IR. 2010. ‘Antifungal Activity of Wood Extract of Aquilaria crassna Pierre ex Lecomte Against Agarwood-Inducing Fungi, Fusarium solani’. Journal od Foresty Research, 7(2): 155-165.

Okwu, D, E., Awurum, A.N., dan Okorunkwo, J.I. 2007. Phytochemical Composition and In Vitro Antifungal Activity Screening of Extracts from Citrus Plants against Fusarium oxysporum of Okra Plant (Hibiscus esculentus). Pest Technology, 1(2): 145-148.

Semangun, H. 2001. Horticultural plant diseases in Indonesia. Yogyakarta: Gadjah Mada University Press.

Suryana, I. 2004. The activity of leaf extract of Piper betle Linn. against Rhizoctonia sp. Skripsi. Bogor: Faculty of Forestry, Bogor Agriculture Institute.

Wahyunigtyas, E. 2008. The effect of Graptophyllum pictum extracts against Candida albicans on acrylic resin denture plates. Indonesian Journal of Dentistry, 15(3): 187-191.

Watson, R.R dan Preedy, V.R. 2007. Bioactive foods in promoting health: probiotics and prebiotics. USA: Academic Press.




DOI: https://doi.org/10.26555/symbion.3510

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